Cellular genomics: which genes are transcribed, when and where?

DNA-DNA Subtractive cDNA Cloning Using o

DNA-DNA Subtractive cDNA Cloning Using oligo(dT)30-Latex and PCR: Identification of Cellular Genes Which Are Overexpressed in Senescent Human Diploid Fibroblasts

✍ E. Hara; T. Yamaguchi; H. Tahara; N. Tsuyama; H. Tsurui; T. Ide; K. Oda 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 552 KB

We previously reported an efficient method for subtractive cDNA cloning using oligo(dT) \()_{30}\)-Latex and polymerase chain reaction (PCR) (E. Hara et al., \(\mathrm{Nu}\) cleic Acids Res. 19, 7097-7104, 1991). The subtraction was performed by hybridization between mRNA of cell type \(B\) and the