A tissue culture system is described for explants of mesenchyme from Ambystoma mexicanum limb regeneration blastemas. Explants were cultured on collagen substrate for 3 weeks in minimal essential medium supplemented with the hormones insulin, thyroxine, somatotropin, and hydrocortisone, plus beef em
Cellular contribution from dermis and cartilage to the regenerating limb blastema in axolotls
โ Scribed by K. Muneoka; W.F. Fox; S.V. Bryant
- Book ID
- 118319609
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 554 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0012-1606
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The presence of a mitogenic activity in limb blastemas of axolotls was detected in crude extracts of blastemas at the mid-bud stage. The mitogenicity of the extracts was estimated from the mitotic index of blastema cells grown for 6 days in the presence of limb blastema extracts, with colchicine pre
Regeneration, the homeostatic ability to maintain tissue structure in the face of normal cell turnover or loss of tissue damaged by trauma or disease, is an essentialย developmental process that continues throughout life. As recently as a decade ago, any serious discussion of the possibility of regen