Cellular and Molecular Procedures in Developmental Biology

✍ Scribed by Flora de Pablo, Alberto Ferrús and Claudio D. Stern (Eds.)

• Publisher: Academic Press
• Year: 1997
• Tongue: English
• Leaves: 357
• Series: Current Topics in Developmental Biology 36
• Edition: 1st
• Category: Library

✦ Synopsis

This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. Key Features * Provides straightforward, current protocols and critical appraisals * Includes diverse analysis of cellular and molecular techniques * Presents everything from whole-organ cultures to electrophysiological approaches * Details a variety of methods for interfering with gene function in various species * Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis

✦ Table of Contents

Content:
Editorial Board
Page ii

Edited by
Page iii

Copyright Page
Page iv

Contributors
Pages xi-xiii

Preface
Pages xv-xvi
Flora de Pablo, Alberto Ferrús, Claudio D. Stern

1 The Avian Embryo as a Model in Developmental Studies: Chimeras and in Vitro Clonal Analysis Original Research Article
Pages 1-35
Elisabeth Dupin, Catherine Ziller, Nicole M. Le Douarin

2 Inhibition of Gene Expression by Antisense Oligonucleotides in Chick Embryos in Vitro and in Vivo Original Research Article
Pages 37-49
Aixa V. Morales, Flora de Pablo

3 Lineage Analysis Using Retroviral Vectors Original Research Article
Pages 51-68,68A,69-74
Constance L. Cepko, Shawn Fields-Berry, Elizabeth Ryder, Christopher Austin, Leffrey Golden

4 Use of Dominant Negative Constructs to Modulate Gene Expression Original Research Article
Pages 75-98
Giorgio Lagna, Ali Hemmati-Brivanlou

5 The Use of Embryonic Stem Cells for the Genetic Manipulation of the Mouse Original Research Article
Pages 99-108,108A,109-114
Miguel Torres

6 Organoculture of Otic Vesicle and Ganglion Original Research Article
Pages 115-131
Juan J. Garrido, Thomas Schimmang, Juan Represa, Fernando Giraldez

7 Organoculture of the Chick Embryonic Neuroretina Original Research Article
Pages 133-140,140A,141-144
Enrique J. de la Rosa, Begoña Díaz, Flora de Pablo

8 Embryonic Explant and Slice Preparations for Studies of Cell Migration and Axon Guidance Original Research Article
Pages 145-159
Catherine E. Krull, Paul M. Kulesa

9 Culture of Avian Sympathetic Neurons Original Research Article
Pages 161-182
Alexander v. Holst, Hermann Rohrer

10 Analysis of Gene Expression in Cultured Primary Neurons Original Research Article
Pages 183-195
Ming-Ji Fann, Paul H. Patterson

11 Selective Aggregation Assays for Embryonic Brain Cells and Cell lines Original Research Article
Pages 197-210
Shinichi Nakagawa, Hiroaki Matsunami, Masatoshi Takeichi, Hiroaki Matsunami

12 Flow Cytometric Analysis of Whole Organs and Embryos Original Research Article
Pages 211-222
José Serna, Belén Pimentel, Enrique J. de la Rosa

13 Detection of Multiple Gene Products Simultaneously by in situ Hybridization and lmmunohistochemistry in Whole Mounts of Avian Embryos Original Research Article
Pages 223-236,236A,237-243
Claudio D. Stern

14 Cloning of Genes from Single Neurons Original Research Article
Pages 245-258
Catherine Dulac

15 Methods for Detecting and Quantifying Apoptosis Original Research Article
Pages 259-278
Nicola J. McCarthy, Gerard I. Evan

16 Methods in Drosophila Cell Cycle Biology Original Research Article
Pages 279-288,288A,289-291
Fabian Feiguin, Salud Llamazares, Cayetano González

17 Single Central Nervous System Neurons in Culture Original Research Article
Pages 293-302
Juan Lerma, Miguel Morales, María de

18 Patch-Clamp Recordings from Drosophila Presynaptic Terminals Original Research Article
Pages 303-312
Manuel Martínez-Padrón, Alberto Ferrús

Index
Pages 313-324