Cell wall localization of dihydroflavonol-glucosideβ-glucosidase in flowers ofPetunia hybrida
✍ Scribed by A. W. Schram; E. J. M. Al; N. Douma; L. M. V. Jonsson; P. Vlaming; A. Kooi; G. J. H. Bennink
- Publisher
- Springer-Verlag
- Year
- 1982
- Tongue
- English
- Weight
- 505 KB
- Volume
- 155
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Limbs of flower buds from Petunia hybrida were investigated for fi-glucosidase activity with dihydroflavonol-glucosides and 4-methyl-umbelliferylfl-D-glucoside as substrates. Dihydroflavonol-glucoside fl-glucosidase is localized in the cell wall. This activity has an acid pH optimum and is also active toward 4-methyl-umbelliferyl-fl-glucoside. Besides this activity a neutral fl-glucosidase is present. This activity is soluble and is not active toward dihydroflavonol-glucosides. Using starch gel electrophoresis it was shown that no difference in fl-glucosidase activity is present between mutants able to convert dihydroflavonols into anthocyanins and mutants accumulating dihydroflavonol-glucosides. It is concluded that flglucosidase activity is not involved in anthocyanin synthesis.