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Cell-wall antigens in mesophyll cells and mesophyll-derived protoplasts of sugar beet: possible implication in protoplast recalcitrance?

✍ Scribed by A. Majewska-Sawka; A. Münster

Publisher
Springer
Year
2003
Tongue
English
Weight
558 KB
Volume
21
Category
Article
ISSN
0721-7714

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Guard-cell protoplasts were isolated by enzymic digestion of the epidermis peeled from the abaxial surface of leaves from Commelina communis L. The protoplasts were separated from mesophyll-cell protoplasts and other contaminants by density-gradient centrifugation, and the purity of the preparations