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Cell volume regulation in cultured cerebellar granule neurons

✍ Scribed by H. Pasantes-Morales; T. E. Maar; J. Morán

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
573 KB
Volume
34
Category
Article
ISSN
0360-4012

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✦ Synopsis

Abstract

Cultured rat cerebellar granule neurons exposed to solutions of reduced osmolarity, responded initially by swelling followed by a regulatory volume decrease (RVD) which is completed within 15 min. Increasing external osmolarity lead to cell shrinking but no evidence of volume regulation was observed within 1 hr. Replacing Na^+^ by choline did not affect RVD whereas N‐methyl‐D‐glucamine accelerated the volume recovery and K^+^ suppressed it completely. The blockade of RVD in high extracellular K^+^ was only observed when chloride and nitrate but not sulfate or gluconate were the accompanying anions. Replacing intracellular Cl^−^, by long incubations with gluconate, markedly inhibited RVD. Removal of extracellular Ca^2+^ or addition of dantrolene which blocks Ca^2+^ released from intracellular stores had no effect on RVD. Increasing extracellular taurine prevented RVD. These results indicate that membrane permeability to K^+^, Cl^−^, and taurine is increased by hyposmolarity and suggest the involvement of these molecules in RVD in granule neurons. © 1993 Wiley‐Liss, Inc.

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