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Cell types in rat liver cultures: their identification and isolation

✍ Scribed by J. W. Grisham

Book ID
104671610
Publisher
Springer
Year
1983
Tongue
English
Weight
1022 KB
Volume
53-54
Category
Article
ISSN
0300-8177

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✦ Synopsis

This paper reviews the various types of cells in the liver in vivo and in hepatic cellular suspensions produced by perfusion of the liver with collagenase solutions. Methods to identify and isolate different types of hepatic cells are discussed. In vitro culture of various types of liver cells is reviewed and the identification of cultured cells is considered.

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✍ P. Roy Walker πŸ“‚ Article πŸ“… 1977 πŸ› John Wiley and Sons 🌐 English βš– 759 KB

## Abstract Parenchymal cells were isolated from adult rat liver with an enzyme perfusion technique. The single‐cell suspension, representing 40–50% of the liver's hepatocytes was suspended in medium and maintained in primary culture for up to four days. The cells were found to carry out glycogen s