Cell-type-specific expression of mouse DNA polymerase β-gene is regulated by silencer elements

✍ Scribed by Masamitsu Yamaguchi; Yuko Hayashi; Kozo Ajiro; Akio Matsukage

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 564 KB
• Volume: 141
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041410225

✦ Synopsis

Nagoya 464, lapdn RNA blot hybridization analysis revealed that the steady-state level of DNA polymerase p-mRNA in mouse neuroblastoma N.1 OTG2 cells was approximately fivefold higher than that in NIHIIT3 cells. In order to examine the function of DNA polymerase p-gene silencers in these two cell lines, we employed a chloramphenicol acetyltransferase (CAT)-transient expression assay using the CAT plasmids containing the silencers linked to various promoter-enhancers, In NIH/3T3 cells, DNA polymerase P-gene silencers effectively repressed the function of its own promoter and those of several other heterologous promoter-enhancers. In contrast, the silencers only marginally affected the CAT expression directed by DNA polymerase p-gene promoter and heterologous promoter-enhancers in NI 8TG2 cells. The extent of the increase of CAT expression by removing silencer elements in NIHI3T3 cells wa3 very similar to the ratio of DNA polymerase P-mRNA content in N18TG2 cells to that in NIH/3T3 cells. These results indicate that cell-type-specific expression of DNA polymerase p-gene is primarily controled by the function of its silencer elements.