𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cell type specific chromosome territory organization in the interphase nucleus of normal and cancer cells

✍ Scribed by Narasimharao V. Marella; Sambit Bhattacharya; Lopamudra Mukherjee; Jinhui Xu; Ronald Berezney

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
554 KB
Volume
221
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Numerous studies indicate that the genome of higher eukaryotes is organized into distinct chromosome territories and that the 3‐D arrangement of these territories may be closely connected to genomic function and the global regulation of gene expression. Despite this progress, the degree of non‐random arrangement remains unclear and no overall model has been proposed for chromosome territory associations. To address this issue, a re‐FISH approach was combined with computational analysis to analysis the pair‐wise associations for six pairs of human chromosomes (chr #1, 4, 11, 12, 16, 18) in the G~0~ state of normal human WI38 lung fibroblast and MCF10A epithelial breast cells. Similar levels of associations were found in WI38 and MCF10A for several of the chromosomes whereas others showed striking differences. A novel computational geometric approach, the generalized median graph (GMG), revealed a preferred probabilistic arrangement distinct for each cell line. Statistical analysis demonstrated that ∼50% of the associations depicted in the GMG models are present in each individual nucleus. A nearly twofold increase of chromosome 4/16 associations in a malignant breast cancer cell line (MCFCA1a) compared to the related normal epithelial cell line (MCF10A) further demonstrates cancer related changes in chromosome arrangements. Our findings of highly preferred chromosome association profiles that are cell type specific and undergo alterations in cancer cells, lead us to propose a probabilistic chromosome code whereby the 3‐D association profile of chromosomes contributes to the functional landscape of the cell nucleus, the global regulation of gene expression and the epigenetic state of chromatin. J. Cell. Physiol. 221: 130–138, 2009. © 2009 Wiley‐Liss, Inc

📜 SIMILAR VOLUMES

The subnuclear organization of histone g
The subnuclear organization of histone gene regulatory proteins and 3′ end processing factors of normal somatic and embryonic stem cells is compromised in selected human cancer cell types
✍ Prachi N. Ghule; Zbigniew Dominski; Jane B. Lian; Janet L. Stein; Andre J. van W 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 341 KB 👁 1 views

## Abstract Human histone gene expression is controlled at the level of transcription initiation and subsequent 3′end processing to generate non‐polyadenylated stem‐loop containing histone mRNAs. Transcription is controlled at the G1/S phase transition by the Cyclin E/CDK2 mediated induction of p22

Association of ecto-5?-nucleotidase with
Association of ecto-5?-nucleotidase with specific cell types in the adult and developing rat olfactory organ
✍ Braun, Norbert; Zimmermann, Herbert 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 822 KB

A unique feature of the olfactory epithelium is its ability to give rise to new sensory neurons throughout life and also following injury. Cells at the basal side of the epithelium serve as neurogenic progenitor cells. The enzyme ecto-5Ј-nucleotidase is expressed at the surface of developing nerve c

Expression of the major group-specific a
Expression of the major group-specific antigen (GS-A) of avian type-C viruses in normal chicken cells and tissues
✍ Antti Vaheri; Erkki Ruoslahti 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 French ⚖ 485 KB 👁 1 views

## Abstract The major group‐specific antigen (gs‐a) of avian type‐C viruses was detected by radioimmunoassay in all tissue samples and cultured cells from leukosts‐free chickens. Visceral tissues of individual embryos contained 7–36 ng antigen per mg protein. The mean values in adult tissues were 2

Differential expression of the o6-alkylg
Differential expression of the o6-alkylguanine-DNA alkyltransferase gene in normal human breast and skin tissue: In situ mapping of cell type-specific expression
✍ Gulzar Wani; Steven M. D'Ambrosio 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 740 KB

## Abstract O^6^‐Alkylguanine is a major toxic, mutagenic, and carcinogenic lesion in cellular DNA that is repaired by O^6^‐alkylguanine‐DNA alkyltransferase (ATase). The expression of this gene is directly related to the cellular sensitivity of alkylating agents, with levels of this protein varyin

Loop domain organization of the p53 locu
Loop domain organization of the p53 locus in normal and breast cancer cells correlates with the transcriptional status of the TP53 and the neighboring genes
✍ Andrea C.S. Goes; David Cappellen; Gilson C. Santos Jr.; Iryna Pirozhkova; Marc 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 847 KB

P53 is a tumor suppressor protein critical for genome integrity. Although its control at the protein level is well known, the transcriptional regulation of the TP53 gene is still unclear. We have analyzed the organization of the TP53 gene domain using DNA arrays in several breast cancer and control