Cell-surface laminin-like molecules and α-D-galactopyranosyl end-groups of cloned strongly and weakly metastatic murine fibrosarcoma cells
✍ Scribed by Ivar Amund Grimstad; Vidar Bosnes
- Book ID
- 102865099
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- French
- Weight
- 663 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Indications from previous work that cancer cell-surface laminin-like molecules and a-D-galactopyranosyl end-groups may contribute to spontaneous metastasis were further investigated. Both moieties are known to mediate cell attachment to various foreign surfaces. Five strongly metastatic and 5 weakly metastatic cell clones from a murine fibrosarcoma were examined for the occurrence of both cell-surface moieties by immunofluorescence flow cytometry and microscopy. None of these clones was rich in laminin-like molecules, which were least strongly expressed by the highly metastatic clones. The a-D-galactopyranosyl end-groups were strongly expressed by all strongly metastatic clones and by 2 weakly metastatic clones, but were only weakly expressed by the other weakly metastatic clones. These results indicate that the laminin-like cell-surface molecules are not necessary for spontaneous metastasis formation. However, the a-D-galactopyranosyl end-groups may be necessary, but are not sufficient for the cancer cells to form metastases. These carbohydrates are known to occur on the laminin-like molecules. The present results show that they must also exist on other cell-surface molecules.