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Cell-Surface-Expressed HIV-1 Envelope Induces the Death of CD4 T Cells during GP41-Mediated Hemifusion-like Events

✍ Scribed by Julià Blanco; Jordi Barretina; Karine F. Ferri; Etienne Jacotot; Arantxa Gutiérrez; Mercedes Armand-Ugón; Cecilia Cabrera; Guido Kroemer; Bonaventura Clotet; José A. Esté


Book ID
112274344
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
523 KB
Volume
305
Category
Article
ISSN
0042-6822

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The envelope glycoprotein (gpl20) of HIV-1 was labeled with fluorescein by using 6- [4,6-dichlorotriazinyl]aminofluorescein. The labeled glycoprotein was found to bind to CD4-positive CEM cells. Monoclonal antibody OKT4a but not OKT4 blocked this binding. Similar specific binding of fluorescein-labe