Cell surface and organelle transport share the same enzymatic properties inReticulomyxa
✍ Scribed by Orokos, Donald D. ;Travis, Jeffrey L.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 55 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0886-1544
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✦ Synopsis
Reticulomyxa transports particulates, like bacterial and algal prey items, bidirectionally along the outside of its pseudopodia. This cell surface transport and intracellular organelle transport can be reactivated in detergent permeabilized cell models [Orokos et al., 1997: Cell Motil. Cytoskeleton]. We have used this unique system to compare cell surface and organelle mechanochemistry in situ in the same reactivated pseudopodia. The ATPase activities of both types of transport were indistinguishable; each displayed identical nucleoside triphosphate specificity, transport ATPase kinetics, and inhibitor sensitivity. Organelle and cell surface transport reactivation required ''hydrolyzable'' adenosine nucleoside triphosphates; neither reactivated with GTP, CTP, UTP, ITP, AMP-PNP, AMP-PCP, or ATP-␥-S. However, other ATP analogues, such as 2Ј-deoxy-ATP and 3Ј-deoxy-ATP and 2Ј,3Ј-dideoxy-ATP, supported the reactivation of organelle and cell surface transport at similar, but markedly reduced, velocities. Both transport processes were inhibited similarly by known inhibitors of dynein ATPases such as erythro-9-(3-[2-hydroxynonyl]) adenine (EHNA) or sodium (Na)-orthovanadate. N-ethylmaleimide (NEM) and ultraviolet (UV) irradiation in the presence of Na-orthovanadate and ATP permanently disabled both transport processes. Organelle and surface transport followed identical Michaelis-Menten kinetics with a calculated K m of 118 µM ATP and a maximum translocation velocity (V max ) of 8.33 µm/sec. These findings strongly suggest that cell surface transport shares the same cytoplasmic dynein motor [Schliwa et al.,