Cell-specific expression of neutral glycosphingolipids in vertebrate brain: Immunochemical localization of 3-O-acetyl-sphingosine-series glycolipid(s) in myelin and oligodendrocytes
✍ Scribed by Somsankar Dasgupta; Narayan R. Bhat; Samuel S. Spicer; Edward L. Hogan; Shigeki Furuya; Yoshio Hirabayashi
- Book ID
- 102383552
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 277 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Abstract
The tissue‐ and cell‐specific expression of three neutral glycosphingolipids, gangliotetraosylceramide (GA1), gangliopentaosylceramide (GalNAc‐GA1), and the novel 3‐O‐acetyl‐sphingosine‐series glycolipid (FMC‐5), were examined with monospecific polyclonal antibodies. Immunohistochemical studies of rodent brain cross‐sections indicated that both GA1 and FMC‐5 antibodies stained myelin. In contrast, GalNAc‐GA1 antibody distinctly stained neurons in cerebral cortex, but only partially delineated Purkinje cells and other neurons in cerebellum. Preliminary studies of mixed glial cultures suggested the following: 1) both FMC‐5 and GA1 antibodies stained oligodendrocytes and oligo progenitors, and 2) GalNAc‐GA1 antibody did not stain any cells in the culture. Because the GalNAc‐GA1 was associated with neurons, we examined the immunoreactivity of GalNAc‐GA1 antibody in primary neuronal cultures. Further studies using primary cultures of rat brain oligodendrocytes, and dissociated cerebellar neuronal cultures indicated that both GA1 and FMC‐5 are specifically expressed by oligodendrocytes, whereas GalNAc‐GA1 is primarily localized in interneurons and to some extent in Purkinje neurons. © 2007 Wiley‐Liss, Inc.