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Cell size distribution as a parameter for the predetermination of exponential growth during repeated batch cultivation of CHO cells

✍ Scribed by Thomas Seewöster; Jürgen Lehmann

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
210 KB
Volume
55
Category
Article
ISSN
0006-3592

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✦ Synopsis

The routine measurement of the cell size distribution of a Chinese hamster ovary (CHO) cell population during a repeated batch process enables the predetermination of exponential growth even 24 h before the population enters the log phase, due to a short but significantly increased cell size during the lag phase. A prolongation of the stationary phase causes to progressive limitation in asparagine, serine, and ethanolamine. Such extended limitation influences the duration of the following lag phase and obviously induces a synchronization of the cell population that can be monitored easily by a fast cell size analyzing technique.

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