✦ LIBER ✦

Cell size and RNA content correlate with cell differentiation and proliferative capacity of rat keratinocytes

✍ Scribed by Martin Poot; Marthe Rizk-Rabin; Holger Hoehn; Jana Henriette Pavlovitch

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 767 KB
Volume: 143
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041430211

No coin nor oath required. For personal study only.

✦ Synopsis

Keratinocytes from rat skin were separated according to their size in a specially designed unit-gravity sedimentation chamber. The tractions obtained with this technique showed clear morphological differences, and analysis of size distribution confirmed that size was the criterion for separation. Simultaneous D N A and RNA staining of the fractions with acridine orange and subsequent flow cytometric analysis enabled one to classify cells into resting, proliferating, and differentiating stages. Cell size was not directly correlated with proliferation in situ as determined with acridine orange flow cytometry, nor with proliferative capacity in culture as assayed by BrdU/Hoechst flow cytometry. The smallest cells, exhibiting low DNA and RNA content, which do not proliferate in vivo, required a prolonged period of serum stirnulation in vitro to initiate RNA and DNA synthesis. Cells of intermediate size exhibited early RNA synthesis and maximal proliferative capacity, whereas the largest cell population displayed no RNA synthesis in culture and the least proliferative capacity. In conclusion, these results suggest that RNA synthesis early after serum stimulation, in addition to a specific, optimal cell size, correlates with the proliferative capacity of keratinocytes in cell culture.

📜 SIMILAR VOLUMES

Content of endoplasmic reticulum and Gol

Content of endoplasmic reticulum and Golgi complex membranes positively correlates with the proliferative status of brain cells

✍ David C. Silvestre; Hugo J.F. Maccioni; Beatriz L. Caputto 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 350 KB

## Abstract Although the molecular and cellular basis of particular events that lead to the biogenesis of membranes in eukaryotic cells has been described in detail, understanding of the intrinsic complexity of the pleiotropic response by which a cell adjusts the overall activity of its endomembran

Expression of the serine protease DESC1

Expression of the serine protease DESC1 correlates directly with normal keratinocyte differentiation and inversely with head and neck squamous cell carcinoma progression

✍ Parish P. Sedghizadeh; Susan R. Mallery; Sarah J. Thompson; Laura Kresty; F. Mic 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 416 KB

## Abstract ## Background. As part of ongoing studies aimed at identifying the molecular events involved in head and neck squamous cell carcinoma progression, we recently isolated a novel serine protease, DESC1. This study was conducted to further characterize DESC1. ## Methods. Specimens of nor

Presence of well-differentiated distal,

Presence of well-differentiated distal, but not poorly differentiated proximal, rat colon carcinomas is correlated with increased cell proliferation in and lengthening of colon crypts

✍ Christopher J. Barnes; W. Elaine Hardman; Ivan L. Cameron 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 119 KB 👁 2 views

To determine whether colon crypt proliferative parameters were significantly altered by the stage of colon carcinogenesis or the type or location of colon tumors in rats, male Sprague-Dawley rats received an injection of the carcinogen 1,2-dimethylhydrazine (12 mg DMH base/kg body weight) or DMH veh

The expression of methyl CpG binding fac

The expression of methyl CpG binding factor MeCP2 correlates with cellular differentiation in the developing rat brain and in cultured cells

✍ Jung, Benjamin P. ;Jugloff, Denis G.M. ;Zhang, Guangming ;Logan, Richard ;Brown, 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 382 KB

## Abstract Mutations in the MeCP2 gene cause Rett syndrome, a neurologic condition affecting primarily young girls. To gain insight into the normal function of MeCP2, we examined its temporal and spatial expression patterns, and immunoreactive prevalence, during late embryonic and perinatal brain