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Cell selection from technology libraries for minimizing power

โœ Scribed by Yumin Zhang; Xiaobo (Sharon) Hu; Danny Z. Chen

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
231 KB
Volume
31
Category
Article
ISSN
0167-9260

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Cell selection strategies for making antibodies from variable gene libraries: trapping the memory pool
โœ Robert E. Hawkins; Greg Winter ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 473 KB

The B cells of immunized animals can be used as a source of variable region (V) gene libraries. Such libraries offer a way of making antibodies directly in bacteria: rearranged V genes are amplified using the polymerase chain reaction, cloned and expressed as soluble fragments in bacteria, and then