Cell phenotype (CD23)-dependent variation in EBV genome copy numbers within lymphoblastoid cell lines (LCL)
✍ Scribed by Vidar Wendel-Hansen; Wen Tao; Mats Ericson; George Klein; Anders Rosen
- Book ID
- 102864242
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- French
- Weight
- 560 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Three Epstein-Barr-virus-transformed lymphoblastoid cell lines (LCL) were analysed on the basis of their CD23 expression. Levels of EBV-DNA were compared in the positive and negative subpopulations. Two lines were further analysed with regard to EBNA, cytoplasmic immunoglobulin (clg) and lytic (EA/VCA) protein expression. Both subpopulations had a similar MHC class4 transcription, but the CD23-subpopulation had a lower plating efficiency and a lower rate of DNA synthesis. In the B6, NADSO and 0467.3 cell lines, CD23-cells contained 2 f 0.2 -6.4 f 3.0 times less EBV DNA than the corresponding CD23+ population. EBNA was expressed in 8 I 2 4.2% -93 t 3.8% of the CD23' cells and in 0 -46 2 8.0% of the CD23cells. No CD23+ cells in 86 or NADSO contained any EAIVCA, while 19 f 2.8% -24 2 4.2% of the CD23-cells were positive for the lytic-cycle-associated antigens. Ofthe CD23-cells, 70 2 8.6% -86 t 6.0% were positive for cytoplasmic immunoglobulin compared to 14.7 t 2.7% -14.9 t 1.8% in the corresponding CD23' population. We have previously shown that only 18% of the clg-positive cells were EBNA-positive in the B6 line compared to 94% in the clg-population. This was open to 2 alternative interpretations: loss of EBV genomes from a fraction of the cells with subsequent differentiation to secretory immunoglobulin production, or down-regulation of EBNA expression in differentiating, EBV-genome-positive cells. Our present findings speak for the first alternative, indicating that a certain proportion of the cells may lose their EBV genomes in both long-established and freshly transformed LCLS. This is accompanied by a reduced percentage of EBNA-positive cells, the disappearance of at least one activation marker (CD23) associated with the virally induced blast transformation, and an increased synthesis of clg.
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