β Scribed by Daniele D'Ambrosio, Francesco Sinigaglia
No coin nor oath required. For personal study only.
Leading cell biologists and immunologists present their most useful and innovative techniques for studying the molecular and cellular basis of cell migration in the inflammatory process. The authors provide a series of focused, cutting-edge techniques proceeding from the in vitro analysis of cell migration and the molecular mechanisms underlying this process, to methodologies for the analysis of cell migration in vivo. Experimental approaches useful in establishing the role of cell migration in the pathogenesis of both acute and chronic inflammatory diseases are emphasized. Methods for the analysis of rapid leukocyte adhesion under flow conditions in vitro are described, which may prove especially fruitful for scientists exploring the molecular mechanisms underlying vascular recognition and leukocyte-endothelium interaction. Each fully tested protocol includes an introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and how to avoid known pitfalls.
π SIMILAR VOLUMES
<p>Cell migration is now well recognized as a critical component of the inflammatory disease process, so that its proper understanding promises to generate both ground-breaking basic discoveries and the development of novel therapeutics. In Cell Migration in Inflammation and Immunity: Methods and Pr
Chemokines and their receptors play a central role in the pathogenesis of numerous acute and chronic inflammatory diseases. This book provides practical protocols for the study of chemokine receptor biology.
<p>Central to the better understanding of both molecular mechanisms and disease, cell migration plays an essential role in a variety of biological processes and is now the subject of intense study using an array of powerful new technologies. In Cell Migration: Developmental Methods and Protocols, re