Cell micro-arrays from surface-attached peptide-polymer monolayers

✍ Scribed by Petersen, Sönke ;Loschonsky, Sidar ;Prucker, Oswald ;Rühe, Jürgen ;Biesalski, Markus

• Book ID: 105365203
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 422 KB
• Volume: 206
• Category: Article
• ISSN: 0031-8965
• DOI: 10.1002/pssa.200880484

✦ Synopsis

Abstract

We introduce the design of a chip for the controlled adhesion of anchorage‐dependent cells. A hybrid copolymer, comprising the peptide cell‐recognition motif RGD, attached to a protein‐repellent poly(dimethylacrylamide) PDMAA polymer is used in a lithography process to implement a chemical microstructure on planar surfaces. The characterization of the surface‐attached film along with a first study of its performance in cell adhesion assays is presented. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)