Cell Membrane Transport: Experimental Approaches and Methodologies

✍ Scribed by Rosa Deves (auth.), David L. Yudilevich, Rosa Devés, Salvador Perán, Z. Ioav Cabantchik (eds.)

• Publisher: Springer US
• Year: 1991
• Tongue: English
• Leaves: 483
• Edition: 1
• Category: Library

✦ Synopsis

Experimental science is a complicated creature. At the head there is a Gordian knot of ideas and hypotheses; behind is the accumulated mass of decades of research. Only the laboratory methods, the legs which propel science forward, remain firmly in touch with the ground. Growth, however is uneven; dinosaurs develop by solid means to give a vast body of results, but few ideas. Others sprint briefly to success with brilliant, though ill-supported, ideas. The problems which this book addresses is to maintain an organic unity between new ideas and the current profusion of innovative experimental tools. Only then can we have the framework on which our research thoughts may flourish. The contributors are outstanding scientists in their respective fields and they record here in a clear manner the methodology with which they perform their experiments. They also illustrate some of their most exciting findings. In all chapters the emphasis is on the critical analysis of the methodology which is often avoided in refereed Journals. These techniques are explained in this book in adequate detail. Each chapter is extensively referenced and contains the most recent material available from author's laboratory at the time of going to press.

✦ Table of Contents

Front Matter....Pages i-xvii
Front Matter....Pages 1-1
Kinetics of Transport: Characterizing the Interaction of Substrates and Inhibitors with Carrier Systems....Pages 3-19
Carrier Kinetics Show How the Sodium Pump Uses ATP to Render Pumping of Both Sodium and Potassium Effective, and Suggest a Model for the Action of the F 0 F 1 ATP-Ases....Pages 21-38
Voltage Dependent Conductances: Gating Currents and Single Channel Recordings....Pages 39-56
Methods of Determining the Routes of Intestinal Water Transport....Pages 57-83
Front Matter....Pages 85-85
Characterization of Transport Systems for Solutes at the Blood Side of Endothelial and Parenchymal Cells by Single Circulation Paired-Tracer Dilution: A Review of Recent Studies....Pages 87-106
Some Inexact Methods for the Analysis of Transport Kinetics Using Dual Tracers....Pages 107-112
Transport Studies in Isolated Enterocytes....Pages 113-130
Cell Culture in the Study of Epithelial Development and Function....Pages 131-141
New Approach to the Study of Transport of Biomolecules by Microcarrier Cell Cultures Perfused in a Column Applying a High Resolution Paired-Tracer Technique....Pages 143-161
Methodological Guide for Studying Epithelial Transport with Isolated Membrane Vesicles....Pages 163-186
Front Matter....Pages 187-187
The Use of Ion-Selective Microelectrodes to Study Cellular Transport Processes....Pages 189-214
Voltage-Dependent Ionic Channels: “Whole-Cell” Recording by Patch-Clamp Techniques....Pages 215-237
Electrophysiological Methods in the Study of Water Transport Across Cell Membranes....Pages 239-254
Front Matter....Pages 255-255
A Practical Introduction to the Use of Intracellular Fluorescent Indicators....Pages 257-272
Biomedical Nuclear Magnetic Resonance Spectroscopy and Transport....Pages 273-296
Proton NMR Studies of Transmembrane Solute Transport....Pages 297-323
Optical and NMR Spectroscopy in the Study of Secretion....Pages 325-337
Front Matter....Pages 339-339
Anion Transport Systems: Continuous Monitoring of Transport by Fluorescence (CMTF) in Cells and Vesicles....Pages 341-367
The Molecular Biology of Sugar Transport Proteins....Pages 369-397
Chemical and Molecular Probes of Nucleoside Transport Mechanisms in Mammalian Tissues....Pages 399-421
Front Matter....Pages 423-423
Regulation of Junctional Intercellular Communication by Tyrosine-Protein Kinases. Role of the Cellular Src Gene and Growth Factor Receptors....Pages 425-436
Single-Channel and Whole-Cell Patch-Clamp Experiments on Gland Cells: Activation of Ion Channels Via Internal Messengers....Pages 437-450
Application of Rapid Dual Tracer Dilution Techniques for the Study of Endothelial Cell Amino Acid Transport in Perfused Microcarrier Cultures....Pages 451-469
Back Matter....Pages 471-484

✦ Subjects

Biochemistry, general; Biotechnology; Animal Anatomy / Morphology / Histology; Plant Sciences