Cell-mediated immune reaction against BK virus-transformed cells

✍ Scribed by Rauno Mäntyjärvi; Henry Karjalainen; Mariitta Laaksonen

• Book ID: 104663533
• Publisher: Springer-Verlag
• Year: 1984
• Tongue: English
• Weight: 584 KB
• Volume: 17
• Category: Article
• ISSN: 0340-7004
• DOI: 10.1007/bf00205493

✦ Synopsis

Syngeneic or allogeneic cells transformed by BK virus (BKV) were used to immunize C57BL/6J mice. After in vitro stimulation, lymphocytes prepared from the spleens of immunized mice were used in in vitro cytotoxicity tests. The results of these tests revealed the presence of a cell surface antigen, presumably corresponding to the viral transplantation antigen, common to all tested BKV- and SV40-transformed cell lines of C57BL/6J origin. An allogeneic cell line transformed by BKV also contained the same antigen. Immunization, i.e., in vivo priming, did not require syngeneic transformed cells, whereas cytolysis was only observed when the virus-specific antigen on target cells was associated with the same H2 haplotype as was expressed by effector cells. An additional unidentified antigen was shared by some of the BKV-transformed cell lines and cell lines transformed by simian adenovirus SA7.