Cell line-specific cell surface components of hypotetraploid ascites mammary carcinoma cells inducing humoral reactions in the syngeneic host
✍ Scribed by Takae Tanino; Michiko Saito; Kohji Egawa
- Book ID
- 102276535
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- French
- Weight
- 481 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Cell‐line‐specific cell surface components were demonstrated on cells of various hypotetraploid ascites cell lines, but not on hyperdiploid cells derived from a mammary carcinoma induced by mammary tumor virus (MTV) in syngeneic C3H/He mice. These substances caused cell‐line‐specific transplantation antigenicity and were identified, together with MTV‐associated substances and tumorassociated embryonic materials, as being the binding sites for tumor cell agglutinating factors found in sera from tumor‐bearing and regressor animals. Particles containing the cell line‐specific substances were released from these cells by hypotonic treatment. They could be purified as a single peak in a sucrose density gradient. They were, however, not dissociated from the MTV‐associated substances. Absorption studies of agglutination activity were extensively used for demonstration of specificity.