Cell kinetic analysis of non-Hodgkin's lymphomas using in vivo iododeoxyuridine incorporation and flow cytometry

✍ Scribed by Martin Erlanson; Jack Lindh; Björn Zackrisson; Göran Landberg; Göran Roos

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 746 KB
• Volume: 13
• Category: Article
• ISSN: 0278-0232
• DOI: 10.1002/hon.2900130405

✦ Synopsis

The aim of this study was to analyse dynamic cell proliferation parameters in non-Hodgkin's lymphomas. Sixty-one patients with newly diagnosed or with recurrent disease were given iododeoxyuridine (IdUrd) intravenously near 4 h prior to tumour biopsy. After staining with an IdUrd reactive antibody and propidium iodide, S-phase fraction (SPF), labelling index (LI), S-phase duration time (7's) and potential tumour doubling time (Tpot) were determined by flow cytometry. Thirty-eight samples, 15 low grade (LGM) and 23 high grade (HGM) malignant lymphomas, were possible to evaluate. Twenty-three cases were excluded due to aneuploidy, insufficient amount of material or technical problems.

Tpot values varied between 0.8-32.9 days (mean 7.0 days). HGM lymphomas had shorter mean Tpot times than LGM lymphomas (4.8 versus 10.4 days, ~~0 . 0 5 ) .

For Ts the range was 4.2-20.1 h (mean 9.1 h), and a difference between the two histological groups was demonstrated with a longer mean Ts for HGM compared with LGM cases (10.0 versus 7.8 h, ~~0 . 0 4 ) .

Tpot showed a negative correlation with SPF (P=0.003), and Ts demonstrated a positive correlation to SPF (p=0.02). The clinical significance of the dynamic cell proliferation parameters studied remains to be clarified, but the interrelationships between TslSPF and Tslmorphologic subtype might be factors of interest for future prognostic studies in malignant lymphomas.