Cell interactions required for transdetermination in the imaginal leg disc ofDrosophila melanogaster
โ Scribed by Chihara, Carol J. ;Gerhart, John C. ;Lee, Li-Wen
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 316 KB
- Volume
- 201
- Category
- Article
- ISSN
- 0022-104X
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โฆ Synopsis
Abstract
Male foreleg imaginal discs from late third instar larvae were cut along radii as designated on a clockface, and the sectors were tested separately for their ability to transdetermine to wing and to regenerate leg structures. The frequency of transdetermination depended strongly on the position of the cuts: discs cut into 12โ6 and 6โ12 fragments or into 12โ9 and 9โ12 fragments gave wing frequently, derived from the 6โ12 and 9โ12 fragments, respectively; whereas discs cut into 6โ11 and 11โ6 fragments transdetermined rarely. Regeneration showed a different pattern, with the 6โ12, 9โ12 and 6โ11 fragments regenerating frequently. Thus, the 6โ11 fragments regenerated but did not transdetermine, indicating the separability of these two phenomena. The 12โ6 and 11โ6 fragments could be induced to transdetermine more frequently by implanting each in combination with a 6โ11 piece. These results suggest that disc cells must interact with other cells not normally their neighbors in order to transdetermine, and that the transdetermining cells may be located in the upper central region of the disc.