Cell-Inspired Materials and Engineering (Fundamental Biomedical Technologies)

✍ Scribed by Dan Ohtan Wang (editor), Daniel Packwood (editor)

• Publisher: Springer
• Year: 2021
• Tongue: English
• Leaves: 255
• Category: Library

✦ Synopsis

This book highlights cutting-edge studies in the development of cell-inspired biomaterials and synthetic materials that manipulate cell functions and provide the next generation with contemporary tools for treating complex human diseases. It explores the convergence of synthetic materials with cell and molecular biology and surveys how functional materials, when patterned with spatial and temporal precision, can be used effectively to maintain cell proliferation and phenotype in vitro, to trigger specific cell functions, and to redirect cell-fate decisions. Human stem cells are a frequently discussed subject in this book. This is an ideal book for students, cell biologists, researchers interested in interdisciplinary research, and biomedical engineers.

This book also:

Highlights successfully developed technologies in cell engineering that make possible new therapeutic development for previously untreatable conditions

Covers topics including bio-inspired micropatterning, DNA origami technology, synthetic NOS inspired by compartmentalized signaling in cells, and light-induced depolarization of the cell membrane

Illustrates in detail the use of stem cells and synthetic scaffolds to model ethically sensitive embryonic tissues and organs

✦ Table of Contents

Preface
Contents
Part I: Fundamental Stem Cell Science for Organ Engineering
Generation of Hepatocytes from Human ES/iPS Cells for Regenerative Medicine
1 Introduction
2 Cell Sources of Hepatocytes
3 Human ES/iPS Cell-Derived Hepatocyte-Like Cells
3.1 Differentiation and Expansion Technology
3.2 Maturation Technology
3.3 Transplantation Technology
Types of Liver-Failure Model Mouse
Engraftment Efficiency
3.4 Therapeutic Effects
4 Liver Tissue Engineering Using Human ES/iPS Cells
4.1 Scaffold Method
4.2 Spheroid-Formation Method
4.3 Cell Sheet-Stratification Method
4.4 Bioreactor Method
4.5 Organoid-Formation Method
4.6 Construction of Human Liver in a Xenograft Model
5 Conclusion
References
Part II: Fundamental Chemistry for Cell-Inspired Materials and Imaging
Construction of Multistep Catalytic Systems in Protein Assemblies
1 Introduction
2 Immobilization of Exogenous Molecules and Nanoparticles Within Protein Assemblies
3 Electron Transfer Reactions in Porous Protein Crystals
4 Catalytic Reactions in Porous Protein Crystals
4.1 Selective Catalytic Reactions in Porous Protein Crystals
4.2 Photocatalytic Systems Constructed in Porous Protein Crystals
5 Tandem Catalytic Reactions in Protein Cages
6 Conclusions
References
Machine Learning and Monte Carlo Methods for Surface-Assisted Molecular Self-Assembly
1 Introduction
2 Self-Assembly of Organic Precursors on Metal Surfaces
3 Kernelized Machine Learning for Model Construction
3.1 Generation of Database
3.2 Energy Prediction
4 Equivalence Class Sampling for Model Predictions
4.1 Equivalence Class Sampling Idea
4.2 Predictions for the Molecular Self-Assembly Process
5 Another Approach: Bayesian Optimization for Model Predictions
6 Conclusions
References
DNA Nanotechnology to Disclose Molecular Events at the Nanoscale and Mesoscale Levels
1 Introduction
2 DNA Origami for Nanostructure Construction
3 Direct Observation and Regulation of Enzyme Reactions in the DNA Nanostructures
3.1 DNA Methylation
3.2 DNA Base-Excision Repair
3.3 DNA Recombination
3.4 Cas9-Induced DNA Cleavage
4 DNA Binding Proteins and RNA Polymerase
4.1 Zαβ Protein Binding to Z-Form DNA
4.2 Cooperative Binding of Sox2-Pax6
4.3 Movement of Photoresponsive Transcription Factor GAL4
4.4 Transcription with RNA Polymerase
5 Direct Observation and Regulation of DNA Structural Changes in the DNA Nanostructure
5.1 G-Quadruplex Formation and Disruption
5.2 G-Quadruplex Formation Using Four-Strand DNA Assembly
5.3 Topological Control of G-Quadruplex and I-Motif Formation
5.4 Triple Helix Formation
5.5 B–Z Transition in the Equilibrium State
6 Direct Observation of Artificial Molecular Systems Using DNA Nanostructures
6.1 Light-Induced DNA Strand Interaction
6.2 Metal Ion-Induced Base Pair Formation
6.3 Zn2+-Dependent DNA Cleavage by DNAzyme
6.4 Riboswitch and Kissing Complexes of RNA
7 Direct Observation of a Mobile DNA Nanomachine on the DNA Origami Surface
7.1 A DNA Motor System Created on a DNA Origami Scaffold
7.2 Single-Molecule Operation of DNA Motor Using the Programmed Instruction
7.3 Photo-Controlled DNA Motor System Constructed on the DNA Origami
7.4 Photo-Controlled DNA Rotator System Constructed on the DNA Origami
7.5 Transcription Regulation System Integrating RNA Polymerase and Genes on the DNA Origami
8 Direct Observation of Assembly of DNA Origami Structures
8.1 Programmed Assembly System Using DNA Origami
8.2 Site-Selective Modification of DNA Origami Scaffold
8.3 Photo-Controlled Assembly and Disassembly of DNA Origami
8.4 Direct Observation of Assembly and Disassembly of Hexagonal DNA Origami on the Lipid Bilayer
8.5 Large-Sized Assembly of DNA Origami and Visualization on a Lipid Bilayer
8.6 Programmed and Hierarchical Self-Assembly of DNA Origami into DNA Origami Frameworks
8.7 Extended DNA Origami Using RNA as Constructing Materials
9 Three-Dimensional DNA Origami
9.1 Observation of Structural Changes in 3D DNA Origami
9.2 Dynamic Conformational Change in Helical DNA Nanotubes
9.3 Transcription Activation Using the Structural Manipulation of DNA Origami
10 Photo-Controlled Devices for Delivery and Molecular Switch
10.1 Photo-Controlled DNA Origami Nanocapsule
10.2 Manipulation of Photo-Controlled DNA Nanocapsule in the Cell
10.3 Photo-Controlled DNA Origami Nanoscissors
10.4 Photo-Controlled Plasmonic Switching Device
11 Single-Molecule Sensing and Manipulation by Optical Tweezers
11.1 Single-Molecule Detection by Force Sensing DNA Origami Device
11.2 Dynamic Configurational Change of Helical DNA Nanotubes
12 Observation of Single-Molecule Dynamics and Biophysics in the DNA Nanocages
12.1 G-Quadruplex in the DNA Nanocages
12.2 Estimation of the Environment Inside the DNA Nanocages
13 Conclusions and Perspectives
References
Part III: Materials and Chemicals for Cell Control
Materials Designed for Biological Nitric Oxide Delivery
1 Introduction
2 NO Donors with Spontaneous Release
3 Assembling NO Donor Molecules into NO Donor Solid-State Materials
4 Photoactive NO Donors (Caged NO Donors)
5 Porous Materials with Photoactive NO Donors
6 Conclusion
References
Designing Biomimicking Synthetic Transcription Factors for Therapeutic Gene Modulation
1 Introduction
2 Natural Transcription Factors: A Brief Introduction
3 Natural DNA-Binding Proteins for Therapeutic Gene Modulation
4 Development of DNA-Based Synthetic Ligands as Synthetic Transcription Factor Mimics
4.1 Gene Regulation Using Designer PIPs Mimicking TF DBDs
4.2 Designer PIPs with DNA Alkylating Agents and Their Bioactivity
4.3 Alteration of the Chemical Architecture of PIPs for Enhanced Bioefficacy
5 Creation of DNA-Based Epigenetic Switches and Their Biological Evaluation
5.1 Distinct DNA-Based Epigenetic Switches for Therapeutically Important TFs
5.2 Next-Generation Synthetic-TF Mimics for Gene Regulation
6 Creation of a Designer PIP for Mitochondrial Gene Modulation
7 Advanced System to Mimic the Synergistic TF Pair–DNA Interaction with Epigenetic Modulating Activity
8 Summary and Outlook
References
Part IV: Physical Methods for Cell Control
Magnetic Nanoparticles and Alternating Magnetic Field for Cancer Therapy
1 Introduction
2 Magnetic Nanoparticles (MNPs)
3 Magnetic Hyperthermia
4 Nanovalves and Controlled Release of Anticancer Drugs
5 Localizing Magnetic Nanoparticles in Living Tissues Using External Magnetic Field
6 Apparatus and Instruments That Generate Alternating Magnetic Fields (AMF)
7 Clinical Potential
8 Challenges Related to MNPs and AMF
References
Light-Control of Cell Membrane Potential and Its Environment
1 Targets for Light Control of Cell Membrane
1.1 Outer Cell Membrane
Mitochondrial Membrane
2 Light-Control Methods for Cell Membrane Potentials and Surrounding Environments
2.1 Photoexcited States of Molecules for Photocontrol
2.2 Light Control with Heat Generation
2.3 Light Control by Photosensitization Generating 1O2
2.4 Light Control by Utilizing Electron Transfer Reactions
3 Conclusions and Perspective
References
Physical Concepts Toward Cell–Material Integration
1 Interfaces: Where Materials Meet Cells
2 Roles of Interfaces in Biology: Why Are Many Functions Confined in 2D?
3 Interplays of Interfacial Forces
4 Views from Surface Free Energy: Physics of Wetting
5 Connecting Two Worlds: Bio & Electronics
6 Other Examples: Biominerals, Antifouling Materials
7 Concluding Remarks and Perspectives
References
Part V: Artificial Environments for Cell Control
Using Stem Cells and Synthetic Scaffolds to Model Ethically Sensitive Human Placental Tissue
1 Introduction
2 The Human Placenta
3 Current Model Systems for the Study of Human Placentation
4 Modeling Human Placentation with Human Embryonic Stem Cells
5 The 3D Modeling Era
6 Modeling Human Placentation in 3D
7 The Future and Implications of Placental-Organoid Culture
References
Nanofiber Extracellular Matrices in Regenerative Medicine
1 Introduction
2 ECM Sources
2.1 Natural or Recombinant ECM Proteins
2.2 Synthetic ECM Polymers
2.3 Decellularized Matrix
3 ECM Nanoengineering
3.1 Nanotechnology for ECM Engineering
Nanolithography
Nanofiber Strategy
3.2 Functionalization
3.3 ECM Screening
4 Nanofiber ECMs for Regenerative Medicine and Tissue Engineering
4.1 Scaled-Up Culture
4.2 Targeted Differentiation
4.3 Transplantation
5 Future Perspectives
References
Correction to: Magnetic Nanoparticles and Alternating Magnetic Field for Cancer Therapy
Index