𝔖 Bobbio Scriptorium
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Cell free synthesis of bacteriophage lambda replication proteins

✍ Scribed by C. Raab; A. Klein; H. Kluding; P. Hirth; E. Fuchs

Book ID
115907569
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
318 KB
Volume
80
Category
Article
ISSN
0014-5793

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Cloning of the replication gene P of bacteriophage lambda: Effects of increased P-protein synthesis on cellular and phage DNA replication
✍ Klinkert, JΓΆrg ;Klein, Albrecht πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 844 KB

A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/