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Cell-free synthesis of alkaline lipase, a glyoxysomal membrane protein, from castor bean endosperm

✍ Scribed by Masayoshi Maeshima; Atsuko Takeuchi; Tadashi Asahi


Book ID
115919591
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
403 KB
Volume
220
Category
Article
ISSN
0014-5793

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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led