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Cell-free production of active E. coli thioredoxin reductase and glutathione reductase

โœ Scribed by Kurtis G Knapp; James R Swartz


Book ID
117106218
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
248 KB
Volume
559
Category
Article
ISSN
0014-5793

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โœ Ana M. Mata; M. Carmen Pinto; Juan Lรณpez-Barea ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 977 KB

The glutathione reductase from E. coli was rapidly inactivated following aerobic incubation of the pure and cell-free extract enzymes with NADPH, NADH and other reductants. The inactivation of the pure enzyme depended on the time and temperature of incubation (t 1/2 = 2 min at 37 degrees C), and was