CELL FRACTIONATION ANALYSIS OF HUMAN CD8 GLYCOPROTEIN TRANSPORT BETWEEN ENDOPLASMIC RETICULUM, INTERMEDIATE COMPARTMENT AND GOLGI COMPLEX IN TISSUE CULTURED CELLS
✍ Scribed by Maria Carmen Erra; Luisa Iodice; Lavinia Vittoria Lotti; Stefano Bonatti
- Book ID
- 102966817
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 591 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
We have set up an analytical cell fractionation procedure to dissect, by a non-morphological method, the anterograde transport of proteins from endoplasmic reticulum, intermediate compartment and Golgi complex in tissue cultured cells. Using this procedure after pulse-chase labelling of cells expressing human CD8 glycoprotein, we obtained results that: (1) support the view that the intermediate compartment is a distinct station in the export from the endoplasmic reticulum to the Golgi complex; and (2) strongly suggests that the O-glycosylation process starts after the intermediate compartment, presumably in the cis-Golgi complex. 1999 Academic Press K: endoplasmic reticulum; intermediate compartment; Golgi complex; membrane traffic.