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Cell cycle traverse and protein metabolism in human NHIK 3025 cells: The role of anchorage

✍ Scribed by Sissel Rogne; Øystein W. Rønning; Ola Myklebost; Per O. Seglen; Erik O. Pettersen

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
577 KB
Volume
125
Category
Article
ISSN
0021-9541

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✦ Synopsis

We have studied the effect of cell anchorage on the human cell line NHlK 3025 in vitro, to see whether the growth regulating effect of cell anchorage primarily affected DNA division cycle or mass growth cycle. It was found that cell to cell anchorage had the same effect on cell cycle progression as anchorage to a solid surtace, which indicates that it is anchorage per se and not cell shape that is important tor growth control in NHlK 3025 cells. When N H l K 3025 cells were grown without attachment to a solid surtace, both GI and cell cycle duration was prolonged by 6 h, which means that the prolonged cell cycle was due to a prolonged GI. During the first part ot the cell cycle the rate of protein synthesis and degradation was constant, and at the same level in cells grown with and without attachment. This means that the prolonged GI was not due to a reduced protein accumulation or mass growth. Towards the end of the cell cycle protein accumulation was reduced. This effect was either d u e to a size control before cell division or a secondary effect of the prolonged GI. We therefore conclude that cell anchorage as a growth regulator primarily affects the DNNcell division cycle.

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