Cell Cycle-Specific Replication of Escherichia coli Minichromosomes
β Scribed by Alan C. Leonard and Charles E. Helmstetter
- Book ID
- 123633107
- Publisher
- National Academy of Sciences
- Year
- 1986
- Tongue
- English
- Weight
- 813 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/27869
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We have followed, by DNA-DNA hybridization, the variation in the number of copies of prophage P1 relative to two chromosomal markers when the doubling time of the host cells is modified by a change in carbon source. The ratio of P1/chromosome terminus undergoes a twofold decrease when the cell doubl
Increased synthesis of DnaA protein, obtained with plasmids carrying the dnaA gene controlled by the heat inducible lambda pL promoter, stimulated initiation of replication from oriC about threefold. The overinitiation was determined both as an increase in copy number of a minichromosome and as an i