Cell cycle dependent regulation of cdc2 mRNA in mouse fibroblasts: Requirement of protein synthesis and of continued mitogenic stimulation
✍ Scribed by Christine Buquet-Fagot; Dominique Fagot; Jan Mester
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 623 KB
- Volume
- 155
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
in the chemically transformed mouse fibroblasts (BP-A31) placed in a serum-free medium, the cdc2 mRNA content decreases in parallel with the cessation of [ 'Hlthymidine incorporation. Extinction of the cdc2 gene expression is also observed in BP-A31 cells overexpressing the human c-myc oncogene. At quiescence, the cdc2 gene expression can be reinduced with serum or with other mitogens such as insulin or 12-0-tetradecanoyl phorbol 13-acetate (TPA). The kinetics of induction is characterized by a lag period which differs according to the mitogen used and reflects the length of the G1 phase (4-6 h with insulin or serum, 9-12 h with TPA). The cdc2 mRNA accumulation is prevented when protein synthesis is blocked with cycloheximide, also if the drug is added at a time when the synthesis of cdc2 mRNA is already under way. Similarly, removal of the mitogen leads to a cessation of the cdc2 mRNA accumulation. These results suggest that the increased expression of the cdc2 gene is mediated by (a) shortlivid, growth factor-regulated protein(s)