Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors

✍ Scribed by Lucio Miele (auth.), Antonio Giordano, Gaetano Romano (eds.)

• Publisher: Humana Press
• Year: 2004
• Tongue: English
• Leaves: 201
• Series: Methods in Molecular Biology 285
• Edition: 1
• Category: Library

✦ Synopsis

The dysregulation of the normal cell cycle underlies an enormous number of pathological conditions, including the neurodegenerative disorders, all cancers, and diabetes. In Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors, expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus on issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies.

✦ Table of Contents

Front Matter....Pages i-xiii
Front Matter....Pages 1-1
Front Matter....Pages 3-21
Front Matter....Pages 23-28
Front Matter....Pages 29-31
Front Matter....Pages 33-36
Back Matter....Pages 37-40
....Pages 41-44

✦ Subjects

Immunology