Cell cycle activity and expression of prohibitin mRNA

✍ Scribed by A. Jane I. Roskams; Varda Friedman; Carla M. Wood; Lisa Walker; Garrison A. Owens; David A. Stewart; Michael S. Altus; David B. Danner; Xiao-Tie Liu; J. Keith McClung

• Book ID: 102883884
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 683 KB
• Volume: 157
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041570211

✦ Synopsis

Prohibitin, a novel intracellular antiproliferative protein, blocks entry into the S phase of the cell division cycle when its rnRNA is microinjected into normal fibroblasts or HeLa cells. To learn more about the interaction between prohibitin and the cell cycle, we studied the effect of microinjecting prohibitin mRNA at different points during the transition from G,, to S phase and analyzed prohibitin mRNA and protein levels in different parts of the cell cycle. The antiproliferative activity of microinjected prohibitin mRNA is high in G,/C, and falls as cells approach S phase. Prohibitin mRNA and protein levels are high in C,, fall with S phase, rise again in G, , and fall in M. Together, these findings suggest that endogenous prohibitin contributes to the control of the G, to S transition in cycling cells in a complex manner, which involves both a transcriptional and posttranslational mechanism.