Cell Cycle activation and ouabain-sensitive ion movements of 3T3 and C3H-10T½ fibroblasts

✍ Scribed by Wenner, Charles E. ;Cheney, John C. ;Tomei, L. David

• Book ID: 102448680
• Publisher: Wiley (John Wiley & Sons)
• Year: 1981
• Tongue: English
• Weight: 469 KB
• Volume: 15
• Category: Article
• ISSN: 0275-3723
• DOI: 10.1002/jsscb.1981.380150207

✦ Synopsis

Abstract

The introduction of either PGF~2α~ (10^−7^ M) or TPA (10^−7^ M) stimulated, ouabain‐sensitive ^86^Rb^+^ influx at 30 min in postconfluent 3T3‐4 mouse fibroblast cultures by 117% and 124%, respectively. Both TPA and PGF~2α~ at these concentrations stimulated the incorporation of ^3^H‐TdR into DNA. TPA had the greatest stimulatory effect, which was similar to that obtained with 10% fetal calf serum. In accord with the idea that modulation of membrane processes such as Na^+^/K^+^ pump activity in fibroblasts may reflect important events related to the initiation of DNA synthesis, it was observed that in both 3T3‐4 and C3H‐1 0T½ cells there were parallel increases in ^3^H‐TdR incorporation and ouabain‐sensitive ^86^Rb^+^ influxes with 10^−7^ M TPA, whereas PGF~2α~ stimulated a significant increase in ^3^H‐TdR incorporation in 3T3‐4 but not C3H‐10T½ cells and only marginal increases in ouabain‐sensitive ^86^Rb^+^ influx in both. Therefore, although there appears to be a close correlation between Na^+^/K^+^ pump activation and subsequent S‐phase entry following TPA stimulation, a similar correlation for PGF~2α~ cannot be confirmed.