Cell Culture

✍ Scribed by P. Michael Conn (Eds.)

• Publisher: Academic Press
• Year: 1990
• Tongue: English
• Leaves: 427
• Series: Methods in Neurosciences 2
• Category: Library

✦ Synopsis

The ability to assess the response of neural cells is an important goal of research in the neurosciences. The means to measure responses in a chemically defined medium is a positive feature of the use of cell culture and is the major reason its use is so important in this discipline.
Techniques for the purification of cells for culture, for large-scale isolation, for assessing differentiated function, and for tracing lineage are included. Many of the methods can easily be adapted to other systems.

Key Features
Convenient benchtop format
Methods presented for easy adaptation to new systems
Comprehensive protocols included for
Preparation and maintenance of primary and continuous cultures
Cell purification
Bulk isolation
Studying pulsatility, growth on immobile carriers, rotation-mediated aggregation, microcultures, Maximow assembly cultures
Assessment of differentiated and functional aspects: endocrine modulation, morphological variants, receptors, intracellular trafficking
* Clonal analysis and lineage analysis

✦ Table of Contents

Content:
Front Matter
Page iii

Copyright page
Page iv

Contributors to Volume 2
Pages ix-xii

Preface
Page xiii
P. MICHAEL CONN

1 - Multiple Approaches to Brain Culture
Pages 3-16
Cheryl F. Dreyfus, Ira B. Black

2 - Culture and Use of Primary and Clonal Neural Cells
Pages 17-33
Stephen D. Skaper, Laura Facci, Daria Milani, Alberta Leon, Gino Toffano

3 - Generation of Astrocyte Cultures from Normal and Neoplastic Central Nervous System
Pages 33-47
Sean Murphy

4 - Isolation of Astrocytes and Schwann Cells for Culture
Pages 47-56
Ramon Lim, E. Peter Bosch

5 - Culture Methods for Oligodendrocyte Cell Lines and Oligodendrocyte–Type 2 Astrocyte Lineage Cells
Pages 56-75
Jane E. Bottenstein, Samuel F. Hunter

6 - Neuropharmacological Analyses in Organotypic Cultures of Spinal Cord and Dorsal Root Ganglia
Pages 75-86
Stanley M. Crain

7 - Isolation, Culture, and Use of Viable Central Nervous System Perikarya
Pages 87-102
Gary R. Dutton

8 - Cerebral Cortical Neurons in Primary Culture and Application to Neuropharmacological Studies
Pages 103-116
Kinya Kuriyama, Seitaro Ohkuma

9 - Preparation of Highly Purified Populations of Neurons, Astrocytes, and Oligodendrocytes
Pages 119-133
Russell P. Saneto

10 - Preparation, Enrichment, and Growth of Purified Cultures of Neurons and Photoreceptors from Chick Embryos and from Normal and Mutant Mice
Pages 134-150
Ruben Adler

11 - Bulk Isolation of Mouse Oligodendrocytes by Gradient Centrifugations
Pages 153-171
Liane Bologa, Norbert Herschkowitz

12 - Bulk Isolation and Culture of Oligodendroglia from Mature Brain
Pages 171-178
William T. Norton, Muhammad Farooq

13 - Measurement of Pulsatile Hormone Release from Perifused Pituitary Cells Immobilized on Microcarriers
Pages 181-194
James R. Hansen, P. Michael Conn

14 - Cultivation of Nerve and Muscle Cells on Microcarriers
Pages 195-209
Abraham Shahar

15 - Monolayer and Three-Dimensional Culture of Rat and Human Central Nervous System: Normal and Malignant Cells and Their Interactions
Pages 210-236
Ole Didrik Laerum, Rolf Bjerkvig

16 - Brain Cell Culture in Rotation-Mediated Aggregates
Pages 236-263
Paul C. Goldsmith, Michael E. Berens

17 - Microcultures of Dissociated Primary Central Nervous System Neurons
Pages 263-275
Efrain C. Azmitia

18 - Long-Term Organotypic Culture of Central Nervous System in Maximow Assemblies
Pages 275-296
C. Dominique Toran-Allerand

19 - Preparation and Use of Morphological Variants to Investigate Neuropeptide Action
Pages 299-315
John S. Ramsdell

20 - In Vivo Tissue Culture: Use of Ectopic Anterior Pituitary Gland to Study Neuropeptide and Steroid Actions on Adenohypophysis
Pages 316-329
Frank J. Strobl, Jon E. Levine

21 - Evaluation of Catecholaminergic Activity in Hypothalamic Cultures
Pages 330-341
Barbara A. Bennett, David K. Sundberg, Mariana Morris

22 - Receptors for Bombesin/Gastrin-Releasing Peptide and Epidermal Growth Factor on Central Nervous System Cells
Pages 341-354
Terry W. Moody, Julie Staley, Mei Lee, Richard M. Kris

23 - Use of Hypothalamic Cell Cultures to Study Role of Diffusible Factors in Phenotypic Expression of Central Nervous System Neurons
Pages 355-371
Andrée Tixier-Vidal, Annie Faivre-Bauman

24 - Microinjectable Probes for Tracing Cell Lineage in Development
Pages 375-392
Duncan K. Stuart, Steven A. Torrence, Gunther S. Stent

25 - Methods for Clonal Analysis and Tracing Cell Lineages of Vertebrate Central Nervous System
Pages 393-406
Marcus Jacobson

Index
Pages 407-424