Cell-cell adhesion in limb-formation, estimated from photographs of cell sorting experiments based on a spatial stochastic model
✍ Scribed by Atsushi Mochizuki; Naoyuki Wada; Hiroyuki Ide; Yoh Iwasa
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 358 KB
- Volume
- 211
- Category
- Article
- ISSN
- 1058-8388
No coin nor oath required. For personal study only.
✦ Synopsis
We developed a new method to estimate the magnitude of differential cell-cell adhesion of two tissues based on the spatial patterns in cell-sorting experiments, and applied it to experimental data on progress-zone cells of avian limb bud at stages 20-26. The change in cell distribution in the experiment was recorded, and statistics q B/B for the degree of cell sorting was calculated from the photographs. Based on extensive computer simulations of spatial Markov processes on a 2-D lattice, we derived a formula for q B/B increasing with time. Using least square fitting, differential adhesion A and cell motility m are estimated from the time series data of q B/B obtained from the experiment. The estimated A was close to 0 (the spatial pattern remained random) if the mixed cells were from two tissues of the same stage. If the mixed cells were from different stages, and the estimated A was positive (cell sorting occurred). Estimated A increased with the difference in the stage number of the two tissues from which the cells were sampled. This result can be explained both by the stage-specific change in adhesion molecules and by a linear increase (or decrease) in the amount of adhesion molecules on cell surface.