Celiac Disease: Methods and Protocols

✍ Scribed by Michael N. Marsh

• Book ID: 127435470
• Publisher: Humana Press
• Year: 2010
• Tongue: English
• Weight: 2 MB
• Series: Methods in Molecular Medicine
• Edition: 1
• Category: Library
• ISBN: 0896036502

✦ Synopsis

With the recent development of powerful bioanalytical techniques, research on gluten sensitivity has entered an exciting new phase. In Celiac Disease: Methods and Protocols, Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world, present a collection of these state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both novice and experienced researchers will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays.Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.