CE-ESI-MS/MS as a rapid screening tool for the comparison of protein–ligand interactions

A rapid and direct method for the determ

A rapid and direct method for the determination of active site accessibility in proteins based on ESI-MS and active site titrations

✍ Norah O'Farrell; Michaela Kreiner; Barry D. Moore; Marie-Claire Parker 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 117 KB 👁 2 views

We have developed an electrospray ionisation mass spectrometry (ESI-MS) technique that can be applied to rapidly determine the number of intact active sites in proteins. The methodology relies on inhibiting the protein with an active-site irreversible inhibitor and then using ESI-MS to determine the