𝔖 Bobbio Scriptorium
✦   LIBER   ✦

cDNA microarray analysis of gene expression in pathologic stage IA nonsmall cell lung carcinomas

✍ Scribed by Haruhiko Nakamura; Hisashi Saji; Akihiko Ogata; Makoto Hosaka; Masaru Hagiwara; Takamoto Saijo; Norihito Kawasaki; Harubumi Kato

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
107 KB
Volume
97
Category
Article
ISSN
0008-543X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

BACKGROUND

The relationship between altered gene expression and tumor progression in lung carcinoma has yet to be characterized. Gene expression in pathologic Stage IA nonsmall cell lung carcinoma specimens was analyzed using a cDNA microarray.

METHODS

Surgical specimens were used for the current study. The pathologic stage was IA (AJCC) in five tumors, IB in two, IIA in one, IIIA in one, and IIIB in one. Seven tumor specimens were adenocarcinomas and three were squamous cell carcinomas. Paired mRNAs from carcinoma cells and normal lung tissue specimens from the same lobe were labeled with different fluorochromes during cDNA probe synthesis in a reverse‐transcription reaction. Both synthesized, labeled cDNA probes were mixed and hybridized to the microarray. The signal intensity of each spot was measured by laser scanner and gene expression was quantified as the tumor‐to–normal fluorescence ratio (T:N ratio). The gene was overexpressed when the T:N ratio was greater than 2.0 and underexpressed when the ratio was less than 0.5.

RESULTS

Overall, 40 (9.4%) of the 425 genes evaluated were overexpressed, and 74 genes (17.4%) were underexpressed. In the 5 Stage IA tumor specimens, 31 (7.3%) genes were overexpressed and 76 (17.9%) were underexpressed. For 30 genes (7.1%), expression was different in Stage IA tumor specimens compared with more advanced tumor specimens.

CONCLUSIONS

The cDNA microarray system showed that numerous alterations of gene expression were present in early‐stage nonsmall cell lung carcinoma specimens. Cancer 2003;97:2798–805. Β© 2003 American Cancer Society.

DOI 10.1002/cncr.11406

πŸ“œ SIMILAR VOLUMES

Clinical significance of caspase-3 expre
Clinical significance of caspase-3 expression in pathologic-stage I, nonsmall-cell lung cancer
✍ Tetsuya Takata; Fumihiro Tanaka; Tomoko Yamada; Kazuhiro Yanagihara; Yosuke Otak πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 French βš– 114 KB πŸ‘ 1 views

Caspase-3 is a cysteine protease that plays an important role in the process of apoptotic cell death. Whereas many studies on the clinical significance of apoptosis in the therapy of malignant tumors have been reported, little has been studied clinically on caspase-3. In the present study, the clini

Analysis of gene expression profiles in
Analysis of gene expression profiles in human HL-60 cell exposed to cantharidin using cDNA microarray
✍ Jun-Ping Zhang; Kang Ying; Zhen-Yu Xiao; Bin Zhou; Qing-Shan Huang; Hong-Mei Wu; πŸ“‚ Article πŸ“… 2003 πŸ› John Wiley and Sons 🌐 French βš– 117 KB

## Abstract Cantharidin is a natural toxin that has antitumor properties and causes leukocytosis as well as increasing sensitivity of tumor cells resistant to other chemotherapeutic agents. There is limited information, however, on the molecular pharmacological mechanisms of cantharidin on human ca

Postoperative serum carcinoembryonic ant
Postoperative serum carcinoembryonic antigen levels in patients with pathologic stage IA nonsmall cell lung carcinoma : Subnormal levels as an indicator of favorable prognosis
✍ Noriyoshi Sawabata; Hajime Maeda; Soichiro Yokota; Shin-ichi Takeda; Masaru Koma πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 105 KB πŸ‘ 1 views

## Abstract ## BACKGROUND Elevated serum carcinoembryonic antigen (CEA) levels are sometimes attributable to the production of CEA by malignant cells, and in turn, the antigen itself can enhance the metastatic potential of malignant cells. The authors speculated that low serum CEA levels might be