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cDNA isolation, expression, and chromosomal localization of the mouse Pcph proto-oncogene

✍ Scribed by Juan A. Recio; Norman Zambrano; Lorena de la Peña; Ciaran Powers; David Siwarski; Konrad Huppi; Vicente Notario

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
243 KB
Volume
26
Category
Article
ISSN
0899-1987

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✦ Synopsis

We report here the isolation and characterization of a cDNA from mouse thymus encoding the murine homolog of the protein product of the Syrian hamster Pcph proto-oncogene. The single open reading frame identified in the cDNA sequence encoded a protein predicted to have 428 amino acids, which shared 93.7% amino acid identity with the Syrian hamster Pcph within the first 412 residues but had a shorter, highly dissimilar C-terminus. Northern and western analyses revealed that Pcph mRNA and protein were widely distributed in mouse embryo and adult tissues, with the highest expression in adults detected in kidney and liver. The mouse Pcph proto-oncogene was mapped by linkage analysis to within 3.3 AE 2.3 cM of Pkch-rs1 on chromosome 12. These data should prove valuable in designing studies to define the cellular function of the Pcph proto-oncogene.

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## Abstract The __PCPH__ proto‐oncogene was identified by its frequent activation in Syrian hamster fetal cells exposed to 3‐methylcholanthrene. We previously isolated human __PCPH__ cDNA and studied its expression in normal human tissues. We report herein the pattern of __PCPH__ expression in norm