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cDNA-AFLP display for the isolation of differentially expressed genes during chicory root development

✍ Scribed by Pascale Goupil; Yonis Soubere Mahamoud; Jérôme Poulain; David Windels; Patrice Crété; Brigitte Huss; Serge Rambour

Book ID
117852196
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
146 KB
Volume
160
Category
Article
ISSN
0176-1617

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We have developed a novel efficient approach, termed differential subtraction display, for the identification of differentially expressed genes. Several critical parameters for the reproducibility and enhanced sensitivity of display, as well as steps to reduce the number of false positive cDNA speci