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Cdk9/Cyclin T1 complex: A key player during the activation/differentiation process of normal lymphoid B cells

✍ Scribed by Giulia De Falco; Eleonora Leucci; Anna Onnis; Cristiana Bellan; Chiara Tigli; Stefan Wirths; Giovanna Cerino; Mario Cocco; Domenica Crupi; Antonio De Luca; Antonio Lanzavecchia; Piero Tosi; Lorenzo Leoncini; Antonio Giordano


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
333 KB
Volume
215
Category
Article
ISSN
0021-9541

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✦ Synopsis


Abstract

Cdk9/Cyclin T1 complex is very important in controlling specific differentiative pathways of several cell types. Limited data are available regarding the expression of Cdk9/Cyclin T1 in hematopoietic and lymphoid tissues. Cdk9/Cyclin T1 expression seems to be related to particular stages of lymphoid differentiation/activation. In this study, we observed that the expression level of Cdk9/Cyclin T1 in vivo increases in memory B cells compared to naïve B cells, and in activated B cells, compared to non‐activated ones. The expression level of the Cdk9/Cyclin T1 complex does not increase in cells induced to differentiate in vitro. In addition, we showed that Cdk9 interacts with E12 and E47, specifically activated during Germinal Center (GC) reaction. Taken together this data suggests an active role for the Cdk9/Cyclin T1 complex during lymphoid differentiation through germinal center reaction. J. Cell. Physiol. 215: 276–282, 2008. © 2008 Wiley‐Liss, Inc.