CD4–c-kit–CD3ϵ–IL-2Rα+ Peyer's patch cells are a novel cell subset which secrete IL-5 in response to IL-2: implications for their role in IgA production
✍ Scribed by Masayuki Kuraoka; Masaaki Hashiguchi; Satoshi Hachimura; Shuichi Kaminogawa
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 384 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
In this study, we examined which cell population contributes to IL‐5 production by Peyer's patch (PP) cells. Thy1.2^–^ fraction of PP cells, but not those of splenocytes, secreted IL‐5 in response to IL‐2. We found that CD3ϵ^–^IL‐2Rα^+^ cells purified from the Thy1.2^–^B220^–^ fraction of PP cells secreted IL‐5 when stimulated with IL‐2. CD3ϵ^–^IL‐2Rα^+^ cells were subdivided into CD4^+^ and CD4^–^ populations or c‐kit^+^ and c‐kit^–^ populations, and only the CD4^–^ and c‐kit^–^ CD3ϵ^–^IL‐2Rα^+^ cells secreted IL‐5 in response to IL‐2. CD3ϵ^–^IL‐2Rα^+^ cells did not express NK cell‐markers and exhibited a lymphoid morphology. We have therefore identified CD3ϵ^–^IL‐2Rα^+^ cells as a unique lymphoid population that are not classified into conventional IL‐5‐producing cell populations, such as T cells, mast cells and NK cells. Depletion of CD3ϵ^–^IL‐2Rα^+^ cells from PP resulted in reduced IL‐5 production. Furthermore, IgA secretion by B cells was increased when PP B cells were cocultured with CD3ϵ^–^IL‐2Rα^+^ cells. Taken together, these results suggest that the novel subset of CD4^–^c‐kit^–^CD3ϵ^–^IL‐2Rα^+^ PP cells are capable of secreting a high level of IL‐5 in response to IL‐2, contribute markedly to IL‐5 production and help IgA secretion by B cells.