CD4+ T lymphocytes mediate colitis in HLA-B27 transgenic rats monoassociated with nonpathogenic Bacteroides vulgatus
✍ Scribed by Frank Hoentjen; Susan L. Tonkonogy; Bi-Feng Qian; Bo Liu; Levinus A. Dieleman; R. Balfour Sartor
- Book ID
- 102263195
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 239 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1078-0998
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✦ Synopsis
Background:
Hla-b27/beta2 microglobulin transgenic (tg) rats develop spontaneous colitis when raised under specific pathogen-free (spf) conditions or after mono-association with bacteroides vulgatus (b. vulgatus), whereas germ-free tg rats fail to develop intestinal inflammation. spf hla-b27 tg rnu/rnu rats, which are congenitally athymic, remain disease free. these results indicate that commensal intestinal bacteria and t cells are both pivotal for the development of colitis in tg rats. however, it is not known if t cells are also required in the induction of colitis by a single bacterial strain. the aim of this study was therefore to investigate the role of t cells in the development of colitis in b. vulgatus-monoassociated hla-b27 tg rats.
Methods:
Hla-b27 tg rnu/rnu and rnu/+ rats were monoassociated with b. vulgatus for 8-12 weeks. cd4(+) t cells from mesenteric lymph nodes (mlns) of b. vulgatus-monoassociated rnu/+ tg donor rats were transferred into b. vulgatus-monoassociated rnu/rnu tg recipients.
Results:
B. vulgatus-monoassociated rnu/+ rats showed higher histologic inflammatory scores and elevated colonic interferon-gamma mrna, cecal myeloperoxidase, and cecal il-1beta levels compared to those in rnu/rnu tg rats that did not contain t cells. after transfer of cd4(+) cells from colitic b. vulgatus-monoassociated rnu/+ tg donor rats, b. vulgatus-monoassociated rnu/rnu tg recipients developed colitis that was accompanied by b. vulgatus-induced ifn-gamma production by mln cells in vitro and inflammatory parameters similar to rnu/+ tg rats.
Conclusions:
These results implicate cd4(+) t cells in the development of colitis in hla-b27 tg rats monoassociated with the nonpathogenic bacterial strain b. vulgatus.