CD3+ WT31− peripheral T lymphocytes lack T44 (CD28), a surface molecule involved in activation of T cells bearing the α/β heterodimer
✍ Scribed by Alessandro Poggi; Cristina Bottino; Maria Raffaella Zocchi; Giuseppe Pantaleo; Ermanno Ciccone; Cristina Mingari; Lorenzo Moretta; Alessandro Moretta
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 581 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
CD3' WT31-peripheral T lymphocytes lack T44 (CD28), a surface molecule involved in activation of T cells bearing the CrJP heterodimer
We have applied two-color fluorescence cytofluorometric techniques to the analysis of the distribution of T44 and CD3 antigens in peripheral blood human lymphoctes. While most CD3' cells co-expressed T44 antigen, a small distinct subset was CD3' T44-(2-10% of CD3' cells). This cell subset also did not react with the WT31 monoclonal antibody (mAb), specific for an alp framework determinant of the T cell receptor (TCR). Lack of T44 antigen expression was also observed in purified CD3' WT31-polyclonal populations that had been cultured in medium containing interleukin 2 (IL2) and as well as > 30 clones expressing the CD3'4-8-WT31-surface phenotype. Immunoprecipitation experiments confirmed that expression of T44 molecules was confined to CD3' WT31' peripheral blood T cells. While conventional CD3' WT31' cells produced IL 2 in response to mAb directed to CD2, CD3 or T44 surface molecules, CD3' WT31-cells did not respond to anti-T44 mAb but released I L 2 following stimulation with anti-CD2 or anti-CD3 mAb. Therefore, assuming that anti-T44 mimicks the effect of a still undefined natural ligand our data suggest that T cells expressing the y-gene surface product may be signalled by stimuli which differ, at least in part, from those acting on CD3' WT31' T lymphocytes.