CD3+, CD56+ non-MHC restricted cytotoxic T lymphocytes in two fraternal aids patients: A case report
✍ Scribed by Michael J. Warzynski; Stephen D. Barbour; Robin T. Winkler-Pickett; John R. Ortaldo
- Book ID
- 102814705
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 590 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
We describe two brothers with hemophilia and AIDS, with an unusually large percentage of CD3+, CD56+ lymphocytes. They experienced no major complications with opportunistic infections (01) and infrequent secondary infections, even though they had nearly 0% CD4 lymphocytes for 3 years. Both patients died in 1991 of progressive cardiomyopathy. The patients' lymphocytes were immunophenotyped by flow cytometry and analyzed for functional cytolytic activity against K 5 6 2 and HIV infected HUT 78 cell lines. Single color CD4 counts were 2-9% for 4 years. Dual color CD4 counts at our laboratory demonstrated 0-1% CD4 for the last 6 months. When CD3+ lymphocytes were examined, both patient 1 and patient 2 demonstrated a significantly higher proportion and absolute number of CD3'Ibright"+ , CD56+ double-positive cells, 47% and 22%, respectively, compared to other HIV-positive children with hemophilia (52%). Functional studies with the K 5 6 2 target cell line demonstrated the highest natural killer (NK) lymphocyte activity in patient 1 that could not be augmented by in vitro addition of 11-2, whereas patient 2 had no NK activity unless IL-2 was added. Functional studies with HIV-infected HUT-78 cells demonstrated patient 2 had cytolytic activity against HIV-infected cells and patient 1 had high nonspecific cytolytic activity even against uninfected HUT-78 cells, whereas controls had minimal activity to HUT-78 cells or HIV-infected HUT-78 cells. The case report raises a speculative question requiring a larger database, whether the anti-HIV activity and/or unusual clinical course without typical 0.1. of some AIDS patients may be related to the presence of CD3"brigh'"+, CD56+ lymphocytes of their immune system.