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Cavity-Filling Mutations Enhance Protein Stability by Lowering the Free Energy of Native State

โœ Scribed by Saito, Minoru; Kono, Hidetoshi; Morii, Hisayuki; Uedaira, Hatsuho; Tahirov, Tahir H.; Ogata, Kazuhiro; Sarai, Akinori


Book ID
126823327
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
147 KB
Volume
104
Category
Article
ISSN
0022-3654

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Stability analysis for the cavity-fillin
โœ Hidetoshi Kono; Minoru Saito; Akinori Sarai ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 281 KB

We have analyzed the effect of cavity-filling mutations on protein stability by means of free-energy calculations based on molecular dynamics simulations to identify the factors contributing to stability changes caused by the mutations. We have studied the DNA-binding domain of Myb, which has a cavi