Caveolin-1 polarization in migrating endothelial cells is directed by substrate topology not chemoattractant gradient
✍ Scribed by Santilman, Virginie ;Baran, JoAnne ;Anand-Apte, Bela ;Fox, Paul L. ;Parat, Marie-Odile
- Book ID
- 102097255
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 405 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0886-1544
- DOI
- 10.1002/cm.20153
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✦ Synopsis
Abstract
Polarization is a hallmark of migrating cells, and an asymmetric distribution of proteins is essential to the migration process. Caveolin‐1 is highly polarized in migrating endothelial cells (EC). Several studies have shown caveolin‐1 accumulation in the front of migrating EC while others report its accumulation in the EC rear. In this paper we address these conflicting results on polarized localization of caveolin‐1. We find evidence for the hypothesis that different modes of locomotion lead to differences in protein polarization. In particular, we show that caveolin‐1 is primarily localized in the rear of cells migrating on a planar substrate, but in the front of cells traversing a three‐dimensional pore. We also show that a chemoattractant, present either as a gradient or ubiquitously in the medium, does not alter caveolin‐1 localization in cells in either mode of locomotion. Thus we conclude that substrate topology, and not the presence of a chemoattractant, directs the polarization of caveolin‐1 in motile ECs. Cell Motil. Cytoskeleton 2006. © 2006 Wiley‐Liss, Inc.