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Cationic lipid-mediated transfection in vitro and in vivo

✍ Scribed by Hoekstra, Sandrine Audouy, Dick

Book ID
120462220
Publisher
Informa plc
Year
2001
Tongue
English
Weight
395 KB
Volume
18
Category
Article
ISSN
0968-7688

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## Abstract ## Background Cationic lipids are at present very actively investigated for gene transfer studies and gene therapy applications. Basically, they rely on the formation of DNA/lipid aggregates via electrostatic interactions between their cationic headgroup and the negatively charged DNA.

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Enhanced in vitro and in vivo cationic lipid-mediated gene delivery with a fluorinated glycerophosphoethanolamine helper lipid
✍ Otmane Boussif; JΓ©rΓ΄me Gaucheron; Caroline Boulanger; Catherine Santaella; Hanno πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 164 KB

## Background: One of the main drawbacks of synthetic, non-viral gene vectors is their relatively low in vivo efficiency when compared with viral vectors. the present paper describes the use of a partially fluorinated glycerophosphoethanolamine (f-pe), a close analog of dope, which, as a helper lip